甘肃陇西黄芩灰霉病病原菌分离鉴定及田间药剂防治

为探明黄芩(Scutellaria baicalensis Georgi)茎基部和根部灰霉病的病原菌,本文于2019—2021年春季对甘肃陇西县采集的黄芩病样进行病原菌的分离培养,运用形态学和内转录间隔区(Internal transcribed spacer, ITS)、甘油醛-3-磷酸脱氢酶(Glyceraldehyde-3-phosphate dehydrogenase,G3PDH)、热激蛋白60(Heat shock protein 60,HSP60)和RNA聚合酶亚基II(The second largest subunit of the nuclear RNA polymerase enzyme II,RPB2)多基因方法鉴定,采用菌丝生长抑制法进行药剂室内毒力测定和田间药剂防治试验。结果表明：引起黄芩灰霉病的病原菌为灰葡萄孢(Botrytis cinerea),春季发病时表现为地上部分枯死不发芽,靠近地表的根表面呈暗绿色水渍状,随后地下根木质部变软呈黄褐色腐烂,病健交界明显;室内毒力测定表明,97%咯菌腈对灰葡萄孢菌的抑菌作用最强,EC₅₀为0.01...     

Cortisol Concentrations in Well‐Regulated Dogs with Hyperadrenocorticism Treated with Trilostane

Background

There are no clear treatment guidelines for dogs with clinically well‐regulated hyperadrenocorticism in which serum cortisol concentrations before and after an ACTH stimulation test performed 3–6 hours after trilostane administration are < 2.0 μg/dL.

Objective

To determine if serum cortisol concentrations measured before (Pre1) and after (Post1) ACTH stimulation at 3–6 hours after trilostane administration are significantly lower than cortisol concentrations measured before (Pre2) and after (Post2) ACTH stimulation 9–12 hours after trilostane administration, in a specific population of dogs with clinically well‐regulated hyperadrenocorticism and Pre1 and Post1 <2 μg/dL.

Animals

Thirteen client‐owned dogs with clinically well‐regulated hyperadrenocorticism and Pre1 and Post1 serum cortisol concentrations <2.0 μg/dL 3–6 hours after trilostane administration.

Methods

Prospective study. Dogs had a second ACTH stimulation test performed 9–12 hours after trilostane administration, on the same day of the first ACTH stimulation test. Cortisol concentrations before and after ACTH stimulation were compared using a paired t‐test.

Results

Cortisol concentrations before (1.4 ± 0.3 μg/dL) and after the first stimulation (1.5 ± 0.3 μg/dL, mean ± SD) were significantly lower than cortisol concentration before the second stimulation (3.3 ± 1.6 μg/dL, P = .0012 each). Cortisol concentration before the first stimulation was also significantly lower than cortisol concentration after the second stimulation (5.3 ± 2.4 μg/dL, P = .0001).

Conclusions and clinical importance

In dogs with clinically well‐regulated, trilostane‐treated, hyperadrenocorticism, and cortisol concentrations <2 μg/dL before and after the first stimulation, a second ACTH stimulation test performed 9–12 hours after treatment can result in higher cortisol concentrations that could support continued trilostane treatment.     

Detecting endotoxin activity in bovine serum using an automated testing system

The aim of the present study was to compare the ability of the commercially available portable test system (PTS^TM) to detect endotoxin activity in bovine serum, with that of the traditional LAL-kinetic turbidimetric (KT) and chromogenic (KC) assays. Prior to testing, serum samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS^TM was not significantly different from that of the traditional LAL-based assays. The results using PTS^TM correlated with those using KT (r²=0.963, P<0.001) or KC assays (r²=0.982, P<0.001). Based on these findings, the PTS^TM could be applied as a simplified system to assess endotoxin activity in bovine serum.     

油茶丰产栽培技术

在林场进行了油茶丰产栽培技术试验 ,林地要选择在山腰 ,土壤要求pH值5.0~6.5,周边环境应无污染 ,具有马尾松、木荷、卷斗青冈等树木的森林.结果表明 :油茶丰产栽培之后 ,苗木成活率可达99 .4% .油茶树木生长良好 ,经过3年的经营管理 ,抚育除草 ,施肥 ,病虫害防治 ,林木生长快速 ,3年的油茶树木生长平均树高1.1m,地径5.1cm,树冠宽幅1.2m,即可结果收成.     

滦河水混凝特性的试验研究

针对滦河水的水质特点 ,分三个水质时期分别进行了混凝小试、不同预处理条件下的中试试验 ,以运行成本、处理水质为前提 ,得出了不同水质时期的建议处理方式 :低温低浊期、常温常浊期采用预加氯工艺 ,高温高藻期使用预臭氧+活性炭工艺.     

壳聚糖/杉木粉/PVC复合材料表面抗菌功能化研究

采用贴膜法评价了添加不同分子量和脱乙酰度壳聚糖的杉木粉/PVC复合材料抗大肠杆菌和金黄色葡萄球菌的性能,并通过X射线光电子能谱(XPS)进行表面元素分析。结果表明:在其他条件完全相同的情况下,添加平均分子量为32 W和脱乙酰度为95%的壳聚糖时,复合材料的表面抗菌效果最佳,且对金黄色葡萄球菌抗菌效果明显优于大肠杆菌;XPS的分析也显示添加壳聚糖后,样品表面出现一定强度N1s的谱峰,且C1和C2的峰面积比从1.87下降至1.82,说明复合样品的表面均匀地聚集了一定数量的壳聚糖,使样品表面具有抗菌功能。     

红椎种子不同方式浸种育苗技术试验

在红椎种子用0．3％的高锰酸钾溶液消毒后,采用不同方式清水浸种和ABT3号生根粉溶液浸种,营养土用红壤、火烧土、泥田土、谷壳、家禽粪、牛大便、钙镁磷等不同配置,经过一定时间发酵形成轻基质营养土,进行了容器育苗技术试验,结果显示：应用ABT3号生根粉溶液浸种的种子育苗平均发芽率,比应用清水浸种的种子育苗平均发芽率提高4．1％,轻基质容器育苗1年生的苗木,应用ABT3号生根粉溶液浸种育苗生长的苗木生长量平均高度、径粗、冠幅,分别比应用清水浸种育苗生长的苗木生长量平均苗高大11．23％、径粗12．50％,苗冠大6．12％。